Technology, Engineering & Agriculture

Zebrafish

A Practical Guide to Husbandry, Welfare and Research Methodology

by Claire Allen, Jean-Philippe Mocho

Description

After securing popularity in home aquariums, zebrafish have also become an essential animal for biomedical research. Ensuring they are adequately cared for in research settings is therefore of the utmost importance and this book is designed to help laboratories develop good welfare and husbandry practices, refine procedural protocols and engage with ethical obligations. A wide range of topics are addressed, starting with husbandry aspects such as environmental enrichment, nutrition, housing conditions, diseases, health monitoring, biosecurity and larval rearing. More experimental topics are then discussed, such as genotyping, ageing, imaging, behavioural studies, experimental design and statistics. Ethical challenges are also considered through the environmental impact of aquatic facilities and the 3Rs - Replacement, Reduction and Refinement - with a focus on anaesthesia, analgesia, euthanasia and severity assessment. Zebrafish are a shoaling species by nature but this book also takes into account their individual needs by providing: - practical advice from experienced zebrafish caretakers; - bridges between data acquisition and fish welfare; and - the latest guidance for reporting, reproducibility and compliance. This book is written for zebrafish facility personnel and for researchers who are aware of the need for robust care for their zebrafish model. It also provides an unparalleled insight for ethics committee members with the intention to provide the best care possible for this ever-important fish.

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Author Biography

Claire is a developmental biologist by training, having studied and worked with multiple model organisms on a variety of research projects since the mid-1990s. Prior to becoming the aquarium manager, Claire primarily worked in the laboratory of Prof. Philip Ingham FRS whose research exclusively involved the zebrafish model. Claire was pivotal in managing the Targeting Induced Local Lesions in Genomes (TILLING) programme at Sheffield, collaborating closely with the Sanger Institute, Cambridge. The work that came out of the TILLING project directly contributed to the calculated shift in research, using the zebrafish model, from developmental biology to modelling disease. In 2008, Claire made a career change which utilised her knowledge of UK legislation and research management to become the aquarium manager. This role drew on her broad experience of research, training and understanding of UK project licences. As a Named Animal Care and Welfare Office, specialising in zebrafish, Claire no

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CABI (CAB International)

CABI (CAB International) is an international, non-profit body improving lives by providing information & scientific expertise to solve problems in agriculture & the environment.

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Bibliographic Information

  • Publisher/Imprint CABI / CAB International
  • Publication Date September 2024
  • Orginal LanguageEnglish
  • ISBN/Identifier 9781800629424 / 1800629427
  • Publication Country or regionUnited Kingdom
  • FormatHardback
  • Primary Price 150 GBP
  • Pages496
  • ReadershipCollege/higher education; Professional and scholarly
  • Publish StatusPublished
  • Dimensions234 X 156 mm
  • Reference Code6729

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